Resin Electron Microscopy

The 4 day workshop organized in collaboration with Leica Microsystems is meant for people that do not have experience with standard preparation techniques for transmission electron microscopy. This workshop is primarily practical in nature, although some topics will be dealt with in lectures. The entire procedure from (live) specimen till stained ultrathin resin section will be covered from start to finish. If you participate most of the time will be spent in the lab working with standard specimens like cultured cells or tissues. Besides embedding in a resin and managing the ultra-microtome, the preparation of tools and other necessities will be covered.

We provide an excellent learning environment. When manual skills like ultrathin sectioning of resin blocks are being trained the participants will have their own ultra-microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual covering the topics of the course.

The preliminary program and a more elaborate list of topics that are addressed in this course can be found here.

Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S, 2x Leica Ultracut T, 1x Leica UC6/FC6 ,1 x Leica UC6, 2x Leica UC7. Leica KMR2/3, Leica AC20, Leica EM AMW, Leica EM TRIM2, Leica EM ACE600, Edwards Auto 306 carbon evaporator, JEOL electron microscope, 2 FEI electron microscopes

Teachers: Cell Microscopy Core: Suzanne van Dijk, Tineke Veenendaal, Corlinda ten Brink, Cilia de Heus, Nalan Liv.
Price: € 1400,- excluding hotel and dinners, including lunches.

For registration and further information: click here.
Participants  can pay online with a credit card, and post-registration they immediately receive their invoice(s).

Registrations are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.

Within a week after the registration you will receive additional information about hotels etc. Cancellation is possible till February 21st 2024, thereafter the course fee has to be paid in full. The CMC reserves the right to cancel this course if less than 8 participants have registered on February 21st 2024.

Cryosectioning and Immuno-Electron Microscopy

The 4 day workshop is organized in collaboration with Leica Microsystems and is meant for people that do not have experience with the Tokuyasu technique. Experience in resin sectioning is not necessary, but is definitely an advantage. The workshop is primarily practical in nature, although some topics will be dealt with in lectures. The entire procedure from (live) specimen till immuno-labeled ultrathin section will be covered from start to finish. Most of the time will be spent in the lab working with standard specimens like cultured cells or tissues. Besides managing the ultra cryo-microtome, the preparation of tools and other necessities will be covered.

We provide an excellent learning environment. When manual skills like ultrathin sectioning are being trained the participants will have their own ultra-cryo microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual covering the topics of the course.
A preliminary program and a more elaborate list of topics that are addressed in this course can be found here.

Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S, 2x Leica Ultracut T, 1x Leica UC6/FC6 ,1 x Leica UC6, 2x Leica UC7. Leica KMR3, Leica AC20, Leica EM AMW, Leica EM TRIM2, Leica EM ACE600, Edwards Auto 306 carbon evaporator, JEOL electron microscopes, 2 FEI electron microscopes.

Teachers & lecturers: Cell Microscopy Core: Suzanne van Dijk,  Corlinda ten Brink, Tineke Veenendaal, Cilia de Heus, Nalan Liv.

Price: € 1400,- excluding hotel and dinners, including lunches. (+ € 350,- for optional extra day)

For registration and further information: click here
Participants  can pay online with a credit card, and post-registration they immediately receive their invoice(s).

Registrations are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.

Within a week after the registration you will receive additional information about hotels etc. Cancellation is possible till February 21st 2024, thereafter the course fee has to be paid in full. The CMC reserves the right to cancel this course if less than 8 participants have registered on February 21st 2024.

Advanced workshop Correlative Light Electron Microscopy

We had to discontinue our annual Electron Microscopy Courses in their hands-on format during the pandemic. We used the break to redesign and restructure the content  of our CLEM course.

We are thankful for your understanding and hope to meet you soon!

In the workshop we offer a balanced “mix” of lectures and practical sessions. The entire workflow from specimen to fluorescent image to immune-labeled ultrathin section will be dealt with. Under guidance of the skilled staff of the Utrecht Cell Microscopy Core Life cell CLEM and Section CLEM including the complete Tokuyasu technique will be practiced and explained to the finest detail. Biological samples and immuno-reagents will be available. The program is meant for people that already have experience in cryosectioning and immunolabeling and want to extend their expertise into CLEM. We provide an excellent learning environment. When manual skills like ultrathin cryo-sectioning are being trained the participants will have their own ultra-cryo microtome (often equipped with a camera) to practice on. The number of participants is limited to 12 people. We will provide you with a practical manual covering the topics of the course.

Available equipment: 2 x Reichert Ultracut E, 3x Leica Ultracut S/ with cryo-attachment, 2x Leica Ultracut T with cryo-attachment, 1x Leica UC6/FC6, 1 x Leica FUC/FC7, Leica AFS. Leica IGL. JEOL electron microscopes, 2 FEI electron microscopes equipped for tomography and supporting equipment.

Teachers & lecturers:
Cell Microscopy Core: Cilia de Heus, Corlinda ten Brink, Suzanne van Dijk, Tineke Veenendaal, Nalan Liv.
Diatome: Helmut Gnaegi. Leica: Jan de Weert.

The course fee includes all immuno-reagents, disposables etc. and lunches. Hotel is not included. The course language is English.
Participants are accepted on a “first-come, first-served” basis therefore we recommend that you contact us at your earliest convenience.

Secretariat Cellbiology
Cell Microscopy Core, Center for Molecular Medicine
University Medical Center Utrecht
Heidelberglaan 100, Rm H02.313
NL – 3584 CX Utrecht
Phone:+31-88.755.65.51
cellmicroscopy.nl