Equipment
Equipment for (immuno)-electron microscopy:
- 1 FEI Tecnai 20 electron microscope
- 1 Fei Tecnai 12 electron microscope
- 1 Jeol 1010 electron microscope
- 3 Leica ultra microtomes
- 5 Leica ultra-cryomicrotomes
- 1 Leica EM ICE high-pressure freezer
- 1 Balzers critical point dryer
- 1 Leica AFS 2 freeze substitution machine with EM FSP
- 1 Leica AC20 automated grid stainer.
- 1 Fei SCIOS FIB SEM scanning electron microscope
- 1 Phenom Pro table top SEM
- 1 Sputter coater
- 1 Edwards Auto 306 Carbon Coater
Technical details of the electron microscopes:
| Microscope | Max acc voltage | Filament | STEM | Cryostage | CCD camera | Plate camera | Special features |
| Jeol 1011 | 100 KV | tungsten | – | + | 2048 x 2048 | + | – |
| FEI Tecnai 12 | 120 KV | LaB6 | + | + | 2048 x 2048 | + | Tomography low dose software |
| FEI Tecnai 20 | 200 KV | LaB6 | – | + | 4096 x 4096 | + | |
| FEI SCIOS FIB SEM | 30 KV | N.Col | – | – | – | – | – |
| Phenom | 20,5 KV | CeB6 | – | – | 2048 x 2048 | – | 14 nm Resolution |
Equipment for Light Microscopy:
- 1 Leica Thunder with Tirf and infinity scanner
- 1 Zeiss LSM700
- 1 ECHO Revolve, color camera and fluorescent microscope
- 1 Cell Insight next high troughput microscope
Technical details light microscopes:
| Optics | LSM 700 | Deltavision | Thunder | CellInsight | Revolve |
| Lens 100x |
| yes, DIC | yes, Tirf |
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| Lens 63x | yes, DIC | yes, DIC | yes, Water |
| yes |
| Lens 40x | yes | yes, DIC | yes, 2x Water, Oil |
| yes |
| Lens 20x | yes | yes, DIC | yes | yes | yes |
| Lens 10x |
|
| yes | yes | yes |
| Lens 4x |
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|
|
| yes |
| Light sources | LSM 700 | Deltavision | Thunder | CellInsight | Revolve |
| Laserlines | 405, 488, 555, 639 | 488, 561 |
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| |
| Krypton lamp |
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|
|
| |
| Mercury lamp | yes |
|
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| |
| Halogen lamp | yes |
| yes | yes | yes |
| Xenon lamp | 405, 488, 568, 647 | ||||
| LED lamp | yes | 405, 488, 551, 647 | 405, 488, 555, 639 | 405, 488, 555, 639 |
| Detectors | LSM 700 | Deltavision | Thunder | CellInsight | Revolve |
| Detector | 2 PMT | Coolsnap HQ + Photometrics EMCCD | Prime 95B sCMOS | CMOS | CMOS + Color camera |
| Max resolution | 2048 x 2048 | 1392 x 1024 + 1024 x 1024 | 1608×1608 | ||
| Frames/sec | 5 | max 30 + | 60 |
| Software / techniques | LSM 700 | Deltavision | Thunder | CellInsight | Revolve |
| Software | Zen2010 | Softworx | LasX | Cellomics | Echo |
(Metamorph) | |||||
| FRAP | no | no | yes | no | no |
| FLIP | no | no | yes | no | no |
| FRET | no | no | yes | no | no |
| time series | yes | yes | yes | no | no |
| 3D | yes | yes | yes | yes | yes |
| Deconvolution | no | yes | yes | no | no |
| Computaional clearing | no | no | yes | no | no |
| Panels | yes | yes | yes | yes | yes |
| Point visiting | yes | yes | yes | yes | no |
| Additional features | LSM 700 | Deltavision | Thunder | CellInsight | Revolve |
| Temp control | no | RT- 40°C | RT- 40°C | no | no |
| CO2 control | no | yes | yes | no | no |
| Autom. Z scan | z stepper | DV Stage | yes | yes | no |
| Motorized stage | yes | yes | yes | yes | no |